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The product was redissolved in a minimal amount of methanol and treated with 5 volumes of acetone to precipitate crystal formation

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The screening method described in our previous study (Poplawska et al., 2020) was applied with the LC and MS parameters described in the Table 1

These modifications result in a longer half-life (approximately 2030 hours) and reduced binding to IGF-binding proteins, making it about three times more potent than native IGF-1

US 2610181, Hayes KJ, "Series of nitrofuran compounds comprising the azomethines of 5-nitro-2-acyl furans with 1-amino-hydantoins", issued 9 September 1952, assigned to Norwich Eaton Laboratories Inc